A simple, rapid, accurate and precise first order derivative spectrophotometric method has been developed for simultaneous estimation of candesartan cilexetil (CAN) and pioglitazone hydrochloride (PIO) in their combined pharmaceutical dosage form. This method utilizes methanol as a solvent. Zero crossing point (ZCP) of candesartan cilexetil and pioglitazone hydrochloride was found to be 242.00 nm and 327.60 nm respectively. Hence, estimation of candesartan cilexetil and pioglitazone hydrochloride was done at 327.60 nm and 242.00 nm respectively. Linearity was observed in the concentration range of 10-50 μg/ml for candesartan cilexetil (r2=0.9995) and 20-100 μg/ml for (r2=0.9994). The results of analysis have been validated statistically and by recovery studies. Developed method was applied to pharmaceutical dosage form. The results were found to be within acceptance criteria according to ICH guideline.
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